The Significance of the Heterogeneity of the Population of Bacteria in Solving Biotechnological Problems by the Example of R-, S- and M-Variants (Dissociates) of Pseudomonas Aeroginosa

Abstract

There were considered molecular, physiological-biotechnical and morphological features of R-. R-, S- and M-dissociates of Pseudomonas Aeroginosa. For all three dissociates, 100 % of the sequence similarity of the fragments of their genes encoding 16S pPNC. Using a number of methods, dissociates compared the ways of using glucose. In R-cells respiration prevailed, in M-cells – fermentation with formation of formic acid, which lead to the ability of cells to grow in the anaerobic conditions, in S-cells observed switching from one glucose pathway to another. The determination of the resistance of bacteria to the action of external factors showed that R-dissociate had selective advantage with an increase in temperature of the growth, UV irradiation and storage, M-dissociate with the lowering of the temperature of cultivation and an increase of the osmotic pressure. The use of sub inhibitory concentrations of substances used to suppress the development of biofilms leads to stimulation of growth of these biofilms increase in the proportion due to advantageous increase in the proportion of thick-walled and rapidly growing R-cells.