ekipЭкология и промышленность РоссииEcology and Industry of Russia1816-03952413-6042ООО "Калвис"10.18412/1816-0395-2022-2-39-43ekip-2004Research ArticleНАУЧНЫЕ РАЗРАБОТКИSCIENTIFIC DEVELOPMENTSОчистка фенолсодержащих сточных вод с применением штамма serratia marcescens МТ9Treatment of Phenol-Containing Wastewater by the Bacterial Strain Serratia marcescens MT9КоробовВ.В.KorobovV.V.

канд. биол. наук, ст. науч. сотрудник

Cand. Sci. (Biol.), Senior Research Fellow

podpiska@kalvis.ruЖуренкоЕ.Ю.ZhurenkoE.Yu.

канд. биол. наук, ст. науч. сотрудник

Cand. Sci. (Biol.), Senior Research Fellow

podpiska@kalvis.ruЖариковаН.В.ZharikovaN.V.

канд. биол. наук, ст. науч. сотрудник

Cand. Sci. (Biol.), Senior Research Fellow

podpiska@kalvis.ruЯсаковТ.Р.IasakovT.R.

канд. биол. наук, ст. науч. сотрудник

Cand. Sci. (Biol.), Senior Research Fellow

podpiska@kalvis.ruМаркушеваТ.В.MarkushevaТ.V.

д-р биол. наук, ст. науч. сотрудник

Dr. Sci. (Biol.), Senior Research Fellow

podpiska@kalvis.ruУфимский институт биологии УФИЦ РАНРоссияUfa Institute of Biology Ufa Federal Research Centre of the Russian Academy of SciencesRussian Federation2022090220222623943Copyright © ООО "Калвис", 20222022ООО "Калвис"ООО "Калвис"https://www.ecology-kalvis.ru/jour/about/submissions#copyrightNoticehttps://www.ecology-kalvis.ru/jour/article/view/2004

Описаны свойства штамма-деструктора фенола и 2,4-дихлорфенола Serratia marcescens МТ9, выделенного из почвы расположенного в г. Уфе (Республика Башкортостан) предприятия, крупного производителя продуктов органической химии в России. Идентификация штамма проведена с учетом культурально-морфологических, физиолого-биохимических и морфометрических признаков, а также результатов сравнительного анализа последовательности гена 16S рРНК. В периодической культуре исследован рост S. marcescens MT9 в условиях использования фенола и 2,4-дихлорфенола в концентрации 100 мг/л в качестве источника углерода и энергии. Установлено, что содержание фенола в культуральной жидкости снижается к 6-м суткам на 82 %, а 2,4-дихлорфенола – к 8-м суткам на 65 % от контроля. Выявлена возможность применения S. marcescens MT9 для утилизации фенола в промышленных стоках нефтехимического производства и производства дубильных экстрактов. Степень очистки сточных вод при использовании штамма marcescens MT9 составила 89,3–99,6 %.

The features of the phenol and 2,4-dichlorophenol-degrading strain S. marcescens MT9, isolated from the soil of the Ufa (Republic of Bashkortostan) a major producer of organic chemistry products in Russia, are described. Identification of the strain was performed according to the cultural, morphological, physiological, biochemical, morphometric features, as well as the results of the comparative analysis of the 16S rRNA gene sequence. The growth of S. marcescens MT9 in the batch culture with phenol and 2,4-dichlorophenol as sole source of carbon and energy in concentration 100 mg/l was studied. It was established that the phenol and 2,4-dichlorophenol content in the culture liquid was reduced to the 6th day by 82 % and to the 8ths day by 65 % from the control, respectively. The possibility of using S. marcescens MT9 for phenol and 2,4-dichlorophenol utilization in industrial wastewater of petrochemical production and production of tanning extracts has been revealed. The level of wastewater treatment using strain S. marcescens MT9 was 89,3–99,6 %.

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The authors declare that there are no conflicts of interest present.